Human Immunodeficiency Virus Type 1 Can Establish Latent Infection in Resting CD4+T Cells in the Absence of Activating Stimuli
- 15 November 2005
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 79 (22) , 14179-14188
- https://doi.org/10.1128/jvi.79.22.14179-14188.2005
Abstract
Resting CD4+ T cells are the best-defined reservoir of latent human immunodeficiency virus type 1 (HIV-1) infection, but how the reservoir is formed is unclear. Understanding how the reservoir of latently infected cells forms is critical because it is a major barrier to curing HIV infection. The system described here may provide an in vitro model of latent HIV-1 infection in resting CD4+ T cells. We demonstrated that HIV-1 integrates into the genomes of in vitro-inoculated resting CD4+ T cells that have not received activating stimuli and have not entered cell cycle stage G1b. A percentage of the resting CD4+ T cells that contain integrated DNA produce virus upon stimulation, i.e., are latently infected. Our results show that latent HIV-1 infection occurs in unstimulated resting CD4+ T cells and suggest a new route for HIV-1 reservoir formation.Keywords
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