Determination of Glutamic Oxalacetic Transaminase Activity by Coupling of Oxalacetate with Diazonium Salts
- 1 March 1967
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 13 (3) , 175-185
- https://doi.org/10.1093/clinchem/13.3.175
Abstract
A method for measuring serum glutamic oxalacetic transaminase activity is described, in which substrate concentrations are more nearly optimal than in previous colorimetric methods. By coupling the diazonium salt at a pH of 4.2, interference from α-oxoglutarate is negligible. Oxalacetate is produced at a linear rate to 180 I.U. Results correlate well with those obtained by a reference spectrophotometric method.Keywords
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