CHOLESTEROL IN HIGH-DENSITY LIPOPROTEIN - USE OF MG2+/DEXTRAN SULFATE IN ITS ENZYMIC MEASUREMENT

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  • 1 January 1978
    • journal article
    • research article
    • Vol. 24  (6) , 931-933
Abstract
A method for measuring human serum high-density lipoprotein cholesterol is described. MgCl2 and dextran sulfate are used to precipitate all low density and very low density lipoproteins. The supernate contains only high density lipoproteins, the cholesterol concentration of which is estimated by an enzymic method, with a discrete analyzer (Abbott Bichromatic Analyzer). Concentration and instrument response are linearly related to 50 mg/l. The precision of the method is excellent in the range of clinical interest (100-1000 mg of cholesterol/l). The precision and efficiency of the precipitation are shown at various concentrations of high-density lipoprotein cholesterol. The method was compared to that of 2 laboratories in the Cooperative Lipoprotein Phenotyping Study group by testing a number of split samples. Agreement was good.