Phosphatidic Acid Synthesis in Castor Bean Endosperm
- 1 March 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 59 (3) , 459-463
- https://doi.org/10.1104/pp.59.3.459
Abstract
Enzyme assays on organelles isolated from the endosperm of castor bean (Ricinus communis cv. ''Hale'') by sucrose density gradient centrifugation showed that palmitoyl-CoA:sn-glycerol 3-phosphate acyltransferase (EC 2.3.1.15) was localized in the membranes of the endoplasmic reticulum. Mn2+ was required for activity, but Ca2+ and Mg2+ could substitute for Mn2+ at higher concentrations. The apparent Km was 170 .mu.M for sn-glycerol 3-phosphate and approximately 8 .mu.M for palmitoyl-CoA. The optimum pH range was 7-7.5, and the principal reaction product was diacyl-sn-glycerol 3-phosphate (phosphatidic acid). Monoacyl-sn-glycerol 3-phosphate (lysophosphatidic acid) was not released as a free intermediate in the reaction. The maximum activity of the enzyme occurred immediately after imbibition, preceding the development of mitochondria and glyoxysomes.Keywords
This publication has 22 references indexed in Scilit:
- Phosphatidylcholine Synthesis in Castor Bean EndospermPlant Physiology, 1976
- Acylation of sn-glycerol 3-phosphate in Escherichia coli. Study of reaction with native palmitoyl-acyl carrier protein.Journal of Biological Chemistry, 1975
- sn-Glycerol-3-phosphate acyltransferase activity in particulate preparations from anaerobic, light-grown cells of Rhodopseudomonas spheroides. Involvement of acyl thiolester derivatives of acyl carrier protein in the synthesis of complex lipids.Journal of Biological Chemistry, 1975
- Pulmonary phospholipid biosynthesis Properties of a stable microsomal glycerophosphate acyltransferase preparation from rabbit lungBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1974
- Acylation of glycerol‐3‐phosphate by rabbit heart mitochondria and microsomes: Triiodothyronine‐induced increase in its activityFEBS Letters, 1974
- Enzymes of Phospholipid Metabolism in the Endoplasmic Reticulum of Castor Bean EndospermPlant Physiology, 1973
- Partial Purification and Properties of an Acyl Coenzyme A:sn-Glycerol 3-Phosphate Acyltransferase from Rat Liver MitochondriaJournal of Biological Chemistry, 1973
- Intracellular Distribution of Enzymes of the Cytidine Diphosphate Choline Pathway in Castor Bean EndospermProceedings of the National Academy of Sciences, 1972
- Influence of sucrose on protein determination by the Lowry procedureAnalytical Biochemistry, 1968
- BIOSYNTHESIS OF LIPIDS IN PLANTS: II. INCORPORATION OF GLYCEROPHOSPHATE-32P INTO PHOSPHATIDES BY CELL-FREE PREPARATIONS FROM SPINACH LEAVESCanadian Journal of Biochemistry, 1966