Analysis of in vitro and in vivo products of the TMV 30kDa open reading frame using antisera raised against a synthetic peptide
Open Access
- 12 December 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 164 (2) , 355-360
- https://doi.org/10.1016/0014-5793(83)80316-0
Abstract
The peptide Tyr‐Ser‐Glu‐Ala‐Thr‐Val‐Ala‐Glu‐Ser‐ASP‐Ser‐Phe (the predicted C‐terminal 11 amino acids of the TMV 30kDa open reading frame plus an additional N‐terminal Tyr residue) was synthesized by solid phase methods and used to raise antisera in rabbits. These antisera precipitated 4 major proteins (p30, p28, p19 and 18.5kDa) from in vitro translation products of TMV short rod RNA , but only one, of apparent M r = 30500, FROM TMV‐infected tobacco protoplasts. This protein was made between 8 and 16 h post infection, and had [35S]Met‐labelled tryptic peptides identical to those of in vitro synthesized p30.Keywords
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