Bone-Inducing Activity of Mature BMP-2b Produced from a Hybrid BMP-2a/2b Precursor
Open Access
- 1 January 1991
- journal article
- other
- Published by The Endocrine Society in Molecular Endocrinology
- Vol. 5 (1) , 149-155
- https://doi.org/10.1210/mend-5-1-149
Abstract
The human osteoinductive proteins BMP-2a and BMP-2b have been cloned and expressed in mammalian cells. In order to improve expression levels we examined the role of the proregion in assembly and export. Use of the BMP-2a proregion combined with the mature region of BMP-2b leads to dramatically improved expression of mature BMP-2b. Mature BMP-2b has been purified to near homogeneity from the BMP-2a/2b hybrid, and its structural properties and biological activity determined. Recombinant mature BMP-2b homodimer elicits bone formation in vivo.Keywords
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