Detection of Avian Oncovirus Group-specific Antigens by the Enzyme-linked Immunosorbent Assay

Publisher Website
Google Scholar
Abstract
A 3-step sandwich enzyme-linked immunosorbent assay [ELISA] was developed for the detection of avian oncovirus group-specific (gs) antigens. The assay procedure was to coat the wells of microtiter plates with hamster anti-gs Ig[immunoglobulin]G, react with crude or purified antigen and finally with hamster anti-gs IgG linked to horseradish peroxidase. The sensitivity was 8 picograms (pg) of input avian myeloblastosis virus (AMV) protein, with negligible background. As the ELISA takes less than 2 h to perform, large-scale screening for infected birds is feasible. A blocking assay was also developed for detecting anti-gs antibodies by adding unlabeled antiserum after the antigen step.