Chitin Synthase in Aspergillus nidulans: Properties and Proteolytic Activation
- 1 March 1977
- journal article
- research article
- Published by Microbiology Society in Journal of General Microbiology
- Vol. 99 (1) , 69-76
- https://doi.org/10.1099/00221287-99-1-69
Abstract
Chitin synthase (EC 2.4.1.16) from membrane preparations of A. nidulans was characterized and the optimum conditions for enzyme activity were determined. A reaction velocity-substrate concentration plot was sigmoidal, but was hyperbolic in the presence of N-acetylglucosamine when the Km for UDP-N-acetylglucosamine was 3.1 mmol l-1. Chitin synthase activity could be increased 6-fold by digestion of enzyme preparations with trypsin for short periods. The trypsin-activated enzyme had altered kinetic and storage properties.This publication has 8 references indexed in Scilit:
- Allosteric proteins and cellular control systemsPublished by Elsevier ,2009
- A particulate chitin synthase from Aspergillus flavus Link: the properties, location, and levels of activity in mycelium and regenerating protoplast preparationsCanadian Journal of Microbiology, 1976
- The activating system of chitin synthetase from Saccharomyces cerevisiae. Purification and properties of the activating factor.Journal of Biological Chemistry, 1976
- Biosynthesis of Chitin in Spores and Growing Cells of Blastocladiella emersoniiJournal of Biological Chemistry, 1967
- The Synthesis of Chitin by Particulate Preparations of Allomyces macrogynus*Biochemistry, 1966
- KINETICS OF REGULATORY ENZYMES - KINETIC ORDER OF YEAST DIPHOSPHOPYRIDINE NUCLEOTIDE ISOCITRATE DEHYDROGENASE REACTION AND A MODEL FOR REACTION1965
- The Synthesis Of Chitin In Cell-Free Extracts Of Neurospora CrassaJournal of Biological Chemistry, 1957
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951