Immunoelectronmicroscopic localization of actin in ionophore-treated boar sperm
- 1 April 1986
- journal article
- research article
- Published by Wiley in Cell Biology International Reports
- Vol. 10 (4) , 231-238
- https://doi.org/10.1016/0309-1651(86)90069-x
Abstract
Actin in ionophore-A23187 treated boar sperm has been localized by indirect immunofluorescence and immunoelectromicroscopy (IEM), using an anti-actin monoclonal antibody. By IEM, after en bloc staining technique and treatment with 15 nm colloidal gold-IgM complex, actin was found associated with the plasma membrane (PM) and the outer acrosomal membrane vesicles and under the PM of the equatorial segment.This publication has 0 references indexed in Scilit: