Characterization of early simian virus 40 transcriptional complexes: late transcription in the absence of detectable DNA replication.
- 1 December 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (12) , 5443-5447
- https://doi.org/10.1073/pnas.74.12.5443
Abstract
Isolation of early viral transcriptional complexes and incorporation in vitro of radiolabeled precursors into nascent RNA permitted an analysis of early SV-40 transcription. Under conditions such that viral DNA replication was undetectable, both early and late SV-40 RNA were synthesized. Apparently, viral DNA replication is not an absolute requirement for late transcription, and late viral RNA is synthesized in SV-40 infected nonpermissive mouse cells. The majority of the early viral transcriptional activity can be solubilized, indicating that a substantial portion of this RNA is transcribed from free rather than integrated templates. Sedimentation analysis of the transcriptional complexes resulted in the detection of 2 separate peaks of activity, suggesting the possibility of 2 distinct types of early SV-40 templates.Keywords
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