Human herpesvirus 6 infection arrests cord blood mononuclear cells in G2phase of the cell cycle

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Abstract
We here report that after infection with human herpesvirus 6A, human cord blood mononuclear cells accumulate in G2/M phase of the cell cycle. Experiments with foscarnet or ultraviolet (UV)‐irradiated virus stocks pointed at an (immediate‐)early, newly formed viral protein to be responsible for the arrest. At the molecular level, p53, cyclin B1, cyclin A and tyrosine15‐phosphorylated cdk1 accumulated after HHV‐6A infection, indicating an arrest in G2. However, no change was observed in the levels of downstream effectors of p53 in establishing a G2 arrest, i.e. p21 and 14‐3‐3σ. We thus conclude that the HHV‐6A‐induced G2 arrest occurs independently of p53 accumulation.