Analysis of the Function of the Photoreceptors Phytochrome B and Phytochrome D in Nicotiana plumbaginifolia and Arabidopsis thaliana
Open Access
- 1 May 2005
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant and Cell Physiology
- Vol. 46 (5) , 790-796
- https://doi.org/10.1093/pcp/pci073
Abstract
To investigate the mechanism of phytochrome action in vivo, NtPHYB, AtPHYB and phyD:green fluorescent protein (GFP) were overexpressed in Nicotiana plumbaginifolia and Arabidopsis thaliana. The expression of 35S:NtPHYB:GFP and 35S:AtPHYB:GFP complemented the tobacco hgl2 and ArabidopsisphyB-9 mutations, whereas the 35S:AtPHYD:GFP only rescued the hgl2 mutant. All three fusion proteins are transported into the nucleus in all genetic backgrounds. These data indicate that AtPHYD:GFP is biologically active and functions as the main red light receptor in transgenic tobacco, and establish an experimental system for the functional analysis of this elusive photoreceptor in vivo.Keywords
This publication has 25 references indexed in Scilit:
- Isolation and Characterization of phyC Mutants in Arabidopsis Reveals Complex Crosstalk between Phytochrome Signaling PathwaysPlant Cell, 2003
- Signaling activities among the Arabidopsis phyB/D/E‐type phytochromes: a major role for the central region of the apoproteinThe Plant Journal, 2003
- Photocontrol of subcellular partitioning of phytochrome‐B:GFP fusion protein in tobacco seedlingsThe Plant Journal, 2000
- Phytochrome E Influences Internode Elongation and Flowering Time in ArabidopsisPlant Cell, 1998
- A deletion in the PHYD gene of the Arabidopsis Wassilewskija ecotype defines a role for phytochrome D in red/far-red light sensing.Plant Cell, 1997
- Tobacco phytochromes: genes, structure and expressionPlant, Cell & Environment, 1997
- The phytochrome gene family in grasses (Poaceae): a phylogeny and evidence that grasses have a subset of the loci found in dicot angiospermsMolecular Biology and Evolution, 1996
- Evolution of the Phytochrome Gene Family and Its Utility for Phylogenetic Analyses of AngiospermsAnnals of the Missouri Botanical Garden, 1995
- The hy3 Long Hypocotyl Mutant of Arabidopsis Is Deficient in Phytochrome B.Plant Cell, 1991
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970