IMMUNOREACTIVE GROWTH HORMONE-RELEASING FACTOR IN HUMAN STALK MEDIAN EMINENCE
- 1 June 1984
- journal article
- other
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 58 (6) , 1197-1199
- https://doi.org/10.1210/jcem-58-6-1197
Abstract
Immunoreactive growth hormone-releasing factor was detected in extracts of acetone preserved human stalk median eminence utilizing an antiserum against human GRF-(1–44)-NH2, isolated from a pancreatic tumor. y, v The antibody is highly specific for the C-terminus of GRF- (1–44)-NH2, and has a cross-reactivity less than 0.02??with GRF-U-40) free acid, a peptide isolated from a different pancreatic tumor. The mean concentration of IR-GRF in 5 pools of 10–15 stalk-median eminence fragments was 57.7±7.2 ng/mg protein (mean±SEM, range 42.5 – 75.8 ng/mg protein). Gel filtration chromatographic analysis showed that 97? of the IR-GRF coeluted with synthetic hpGRF-(1–44)-NH2 and eluate inhibition of binding in the radioimmunoassay was parallel to that of hpGRF-(1–44)-NH2- These data strongly suggest that human hypothalamic tissue contains a peptide that is similar to hpGRF-( 1–44)-NH2.Keywords
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