A Method for Measurement of Urinary Deoxyribonuclease Based on the Reaction of Indole with Deoxyribonucleotides.

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Abstract
The indole reaction method for the estimation of deoxyribonucleic acid has been modified to determine the activity of urinary deoxyribonuclease I and II. (DNase I and II.) Good repro-ducibility has been assured by the quantitative precipitation of unreacted DNA by adding a protein such as casein or bovine serum albumin to the enzyme reaction mixture. The reaction of indole with the deoxyribonucleotides, combined with the failure of the corresponding nucleosides or free deoxyribose to react, affords a basis for the quantitative measurement of the deoxyribonucleotides in the presence of the corresponding phosphate free compounds.