Determination of Anabolic Steroid 19-Nor-Testosterone in Bovine Serum by GC-SIM--MS
- 1 October 1993
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Chromatographic Science
- Vol. 31 (10) , 429-432
- https://doi.org/10.1093/chromsci/31.10.429
Abstract
Anabolic steroids extracted from bovine serum by means of organic solvents or solid-phase extraction techniques with C18 cartridges are not suitable for quantitative analysis by gas chromatography—mass spectrometry (GC—MS); therefore, they were extracted from serum by Extrelut columns (Merck; Germany), eluted with diethyl ether, and purified by a solid-phase extraction technique using amino (NH2) columns. The resulting clean extract was treated with heptafluorobutyric anhydride (HFBA); this formed a derivative suitable for GC—MS analysis. Using selected ion monitoring (SIM), quantities as small as 40 pg/mL can be detected.Keywords
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