Immunoelectrophoresis on Blood Serum Proteins of Guinea Pigs with Experimental Amebiasis

Publisher Website
Google Scholar
Abstract
A macro immunoelectro-phoretic technique which uses a thin agar film (0.5 mm) and medium sized plates (4 x 15 cm) is described. By use of agar block bridges and petroleum ether coolant, the electrophoretic step requires only 60 minutes. Due to the thin agar film, steps of eluting the unreacted proteins and of staining are quickly achieved. An economical method. for accurately casting the thin agar films is presented. Immuno-electrophoretic observations were made on serum proteins of 12 guinea pigs inoculated intracaecally with a virulent strain of Entamoeba histolytica and on 6 guinea pigs inoculated with an avirulent strain. Sera from 5 of the 6 guinea pigs with severe or moderate infections of the virulent organisms showed a precipitation pattern in the region of the alpha-1 globulin not observed in other animals or in the same animals before infection. In 10 animals, a precipitin arc, apparently unrelated to infection, was noted in the albumin region.

This publication has 0 references indexed in Scilit: