Detection of Chlamydia trachomatis in rapidly produced McCoy cell monolayers.

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Abstract
The 24 h delay between seedling coverslips with cells and inoculating samples for culture of Chlamydia was reduced to less than 1 h by using coverslips which had been pre-treated with glutaraldehyde-activated .gamma.-aminopropyltriethoxysilane. Treated coverslips were not toxic for McCoy [female synovial] cells and even 1 yr after treatment monolayers formed rapidly on them. All of 13 C. trachomatis serotypes and 1 C. psittaci strain tested produced inclusions in such cell monolayers. In comparative tests, when there were large numbers of inclusions, more were always seen in conventionally produced monolayers than in monolayers on treated coverslips. When there were few inclusions, more were seen in the latter monolayers, a phenomenon observed with unpassaged Chlamydia in clinical specimens and in laboratory-passaged strains. The rapid method is as sensitive for isolating Chlamydia as conventionally produced monolayers.