Multiple 3' ends of the chicken pro α2(1) collagen gene

Open Access

1 January 1983

journal article
Published by Oxford University Press (OUP) in Nucleic Acids Research

Vol. 11 (16) , 5443-5450
https://doi.org/10.1093/nar/11.16.5443

Abstract

The precise location of the 3' ends of the chicken pro α2(I) collagen gene have been identified by SI nuclease protection of overlapping genomic fragments by calvaria poly A containing RNA and size determination of the protected fragments on DNA sequencing gels. The gene ends 300 and 306 bp and 754 and 777 bp from the translation stop codon. The two sets of ends explain the major and minor pro α2(I) collagen mSKAs previously observed, which may result from either RNA polymerase readthrough of the first termination site and/or different processing sites.

Keywords

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