VVA-labelled cells in monkey visual cortex are double-labelled by a polyclonal antibody to a cell surface epitope
- 1 April 1992
- journal article
- Published by Springer Nature in Journal of Neurocytology
- Vol. 21 (4) , 244-259
- https://doi.org/10.1007/bf01224759
Abstract
The staining patterns produced by the lectinVicia villosa and by a commercially available polyclonal antibody generated to substance P were analysed and compared in monkey visual cortex at the light and electron microscopic levels.Vicia villosa lectin labels the cell surface of a subpopulation of cortical cells, producing a meshlike pattern over the soma and proximal dendrites. The polyclonal antibody labels three distinct elements in the cortex: a pericellular epitope present on a subpopulation of non-pyramidal cells, and putative intracellular sites in a type of small pyramidal cell located at the layer 5/6 border, and in a small number of non-pyramidal cells in the underlying white matter. Because of the similarity of the appearance of theVicia villosa lectin labelling and the pericellular labelling produced by the polyclonal antibody, further experiments were conducted to determine the relationship between the cell surface sites recognized by these markers. Double-labelling experiments show that both sites are present on the same population of cells, and at the ultrastructural level both markers appear to outline the intersynaptic cell membrane, sometimes extending around presynaptic elements. However, preadsorption experiments indicate that the markers recognize different sites on the cell membrane. Preadsorption experiments also show that the pericellular epitope recognized by the polyclonal antibody is unlikely to be substance P, but it may be structurally similar to keyhole limpet haemocyanin. Comparison of cortical and subcortical staining patterns produced with the polyclonal antibody and with a commonly used monoclonal antibody to substance P reveal that one of the putative intracellular epitopes recognized by the polyclonal antibody is likely to be substance P.Keywords
This publication has 44 references indexed in Scilit:
- Morphological types of projection neurons in layer 5 of cat visual cortexJournal of Comparative Neurology, 1990
- Early postnatal development of vasoactive intestinal polypeptide‐ and peptide histidine isoleucine‐immunoreactive structures in the cat visual cortexJournal of Comparative Neurology, 1989
- Molecular determinants of GABAergic local-circuit neurons in the visual cortexTrends in Neurosciences, 1989
- The LectinVicia VillosaLabels a Distinct Subset of GABAergic Cells in Macaque Visual CortexVisual Neuroscience, 1989
- Functional implications of the anatomical organization of the callosal projections of visual areas V1 and V2 in the macaque monkeyBehavioural Brain Research, 1988
- Dendritic morphology and axon collaterals of corticotectal, corticopontine, and callosal neurons in layer V of primary visual cortex of the hooded ratJournal of Comparative Neurology, 1988
- Immunohistochemical localization of a membrane-associated, 4.1-like protein in the rat visual cortex during postnatal developmentJournal of Comparative Neurology, 1988
- Efferent systems of primary visual cortex: A review of structure and functionBrain Research Reviews, 1983
- Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.Journal of Histochemistry & Cytochemistry, 1981
- Anatomy of interhemispheric connections in the visual system of Boston Siamese and ordinary catsJournal of Comparative Neurology, 1977