Characterization of nitric oxide synthases in non‐adrenergic non‐cholinergic nerve containing tissue from the rat anococcygeus muscle

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Abstract
Tissue homogenates prepared from rat anococcygeus muscle converted l-arginine to l-citrulline indicating the presence of nitric oxide (NO) synthase. NO synthase activity was also found in crude and partially-purified soluble and particulate fractions prepared from the homogenates. Both soluble and particulate NO synthase were dependent on NADPH, 5,6,7,8-tetrahydrobiopterin and calcium, and inhibited by NG-nitro-l-arginine. Tissue homogenates or crude cytosolic and membrane fractions from rat vas deferens, which does not contain NO releasing non-adrenergic non-cholinergic neurones, had no NO synthase activity.

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