A putative ATP-binding protein from theche/flalocus ofBacillus subtilis
- 1 January 1994
- journal article
- research article
- Published by Taylor & Francis in DNA Sequence
- Vol. 4 (4) , 271-275
- https://doi.org/10.3109/10425179409020852
Abstract
A majority of the chemotaxis and flagellar genes of Bacillus subtilis are found in the major che/fla operon which spans over 26 kilobases of DNA and encodes at least 30 genes. In this operon, a single open reading frame, designated orf298, has been demonstrated to encode a 33, 131 Dalton protein which shows no evidence of being involved in chemotaxis or motility. A strain disrupted in orf298 was assayed for motility and chemotaxis and always behaved as the wild-type strain. Inspection of the translated sequence revealed a consensus ATP-binding region. While the role of ORF298 has not yet been determined, ATP-binding and/or hydrolysis is a likely function.Keywords
This publication has 8 references indexed in Scilit:
- flhF, a Bacillus subtilis flagellar gene that encodes a putative GTP‐binding proteinMolecular Microbiology, 1992
- Bacillus subtilis chemotaxis: a deviation from the Escherichia coli paradigmMolecular Microbiology, 1992
- Transcriptional organization of a cloned chemotaxis locus of Bacillus subtilisJournal of Bacteriology, 1990
- GTP-binding domain: three consensus sequence elements with distinct spacing.Proceedings of the National Academy of Sciences, 1987
- Rapid and Sensitive Protein Similarity SearchesScience, 1985
- A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.Proceedings of the National Academy of Sciences, 1985
- Complete nucleotide sequence of the Azotobacter vinelandii nitrogenase structural gene clusterGene, 1985
- Bacillus subtilis Requires a "Stringent" Shine-Dalgarno Region for Gene ExpressionDNA, 1984