DNA adducts in lymphocytes and granulocytes of smokers and nonsmokers detected by the 32P-postlalbelling assay

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Abstract
The effects of smoking and DNA adduct formation were analysed in isolated human white blood cell populations. As the white cells are composed mainly of granulocytes with a short half-life and T-lymphocytes with a half-life of several years, we isolated the lymphocytes and granulocytes of 11 smokers and 10 nonsmokers to determine any smoking-related DNA adducts by the nuclease-P1-enhanced 32P-postlabelling assay. The differences between the mean lymphocyte DNA adducts/108 nucleotides of 31 ± 5.7 (SE) of smokers were significantly higher (P < 0.05) than those in the lymphocytes 13 ± 1.6 (SE) of nonsmokers. The total DNA adducts/108 nucleotides obtained from the granulocytes of smokers and nonsmokers was 9.6 ± 1.9 and 7.6 ± 1.9 respectively. The plasma cotinine concentrations were hi good agreement with the smoking information given by the individual smokers (r = 0.847, P < 0.001). The DNA adduct levels of the lymphocytes of the 10 smokers correlated with the plasma cotinine concentrations (r = 0.639, P < 0.05). The variation between the results was explained by the variation among the individuals and the samples, but not by the variation hi the parallel determinations. More detailed studies are needed to analyse the source of the individual variations between the smokers' adduct levels, DNA repair, and differences in the metabolism of the compounds in cigarette smoke.

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