Relative and absolute numbers of E- and EAC rosette-forming cells in lymphoid organs
- 1 March 1977
- journal article
- research article
- Published by Springer Nature in Annals of Hematology
- Vol. 34 (3) , 201-210
- https://doi.org/10.1007/bf00996169
Abstract
The percentage of lymphocytes of the blood and lymphoid organs of young pigs were determined by forming rosettes with pretreated sheep red blood cells (AET-rosettes) or with sheep red blood cells coated with antibody and complement (EAC-rosettes). The mean figures for AET-rosettes were 70% for the blood, 93% for the thymus, 78% for lymph-nodes and 68% for the spleen. For EAC-rosettes the numbers were 16%, 0%, 16% and 19% respectively. The absolute numbers of rosette-forming lymphocytes were estimated on the basis of previously determined numbers of lymphoid cells in young pigs. Even by excluding the data for the thymus, there would be about three times more AET-rosette forming lymphocytes than EAC-rosette forming cells in the whole animal. About 11% of all lymphocytes did not show any of these markers. Data on the numbers of “B” and “T” lymphocytes in human lymphoid organs from the literature were summarized and compared to those found in pigs and thus, the absolute numbers for a young adult man estimated. Es wurden die prozentualen Anteile von Lymphozyten im Blut und den lymphatischen Organen des jungen Schweins bestimmt, die mit vorbehandelten Schaferythrozyten (AET-Rosetten) oder mit Antikörper- und Complement-beladenen Schaferythrozyten (EAC-Rosetten) Rosetten bildeten. Die Mittelwerte für die Spontanrosetten (AET) waren 70% für Blutlymphozyten und für Lymphozyten aus dem Thymus 93%, aus Lymphknoten 78% und aus der Milz 68%. Die entsprechenden Werte für EAC-Rosetten betrugen 16% (Blut), 0% (Thymus), 16% (Lymphknoten) und 19% (Milz). Mit Hilfe der früher bestimmten Gesamtzahl von Lymphozyten konnten die Absolutzahlen von E- und EAC-Rosetten-bildenden Lymphozyten ermittelt werden. Auch wenn die Thymuslymphozyten nicht berücksichtigt wurden, so wurden für den Gesamtorganismus eine etwa 3mal höhere Anzahl an AET- als an EAC-Rosettenbildenden Zellen errechnet. Etwa 11% aller Lymphozyten hatten keinen dieser beiden Marker. Angaben aus der Literatur über „T“- und „B”-Lymphozyten in den lymphatischen Organen des Menschen wurden zusammengefaßt, mit den Daten für Schweine verglichen und die Absolutzahlen von Rosetten bildenden Lymphozyten in lymphatischen Organen eines jungen Erwachsenen geschätzt.Keywords
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