ENZYMIC COLOR DEVELOPMENT OF URINARY 3-ALPHA-HYDROXYSTEROIDS ON THIN-LAYER CHROMATOGRAMS
- 1 January 1980
- journal article
- research article
- Vol. 26 (3) , 491-493
Abstract
The use of 3.alpha.-hydroxysteroid dehydrogenase (EC 1.1.1.50) is described for enzymic color development of 3.alpha.-hydroxysteroids. Urinary 3.alpha.-hydroxysteroids are converted to 3-ketosteroids by the enzyme, and the NADH formed is reacted with 2-p-iodophenyl-3-p-nitrophenyl-5-phenyltetrazolium chloride and diaphorase to form the colored formazan. The dye so formed on a TLC plate is measured by densitometric scanning. Use of this method is illustrated by showing results for urinary excretion of 3.alpha.-hydroxysteroids by some various sorts of patients.This publication has 2 references indexed in Scilit:
- Enzymatic reactions on thin-layer chromatographic platesJournal of Chromatography A, 1979
- Separation of Six Urinary 17-Ketosteroids by Two-Dimensional Thin-Layer Chromatography; Control Values and Response to ACTHJournal of Clinical Endocrinology & Metabolism, 1964