Solid Phase Synthesis of a 31-Residue Fragment of Human Glucose-Dependent Insulinotropic Polypeptide (GIP) by the Continuous Flow Polyamide Method.
- 1 January 1987
- journal article
- research article
- Published by Danish Chemical Society in Acta Chemica Scandinavica
- Vol. 41b (7) , 494-498
- https://doi.org/10.3891/acta.chem.scand.41b-0494
Abstract
A fragment, GIP1-31, of the human Glucose-dependent Insulinotropic Polypeptide (GIP1-42) Tyr-Ala-Glu-Gly-Thr-Phe-Ile-Ser-Asp-Tyr-Ser-Ile-Ala-Met-Asp-Lys-Ile-His-Gln-Gln-Asp-Phe-Val-Asn-Trp-Leu-Ala-Gln-Lys-Gly has been synthesized by solid phase methodology under continuous flow conditions. The peptide was assembled on a polydimethylacrylamide kieselguhr support by using 9-fluorenylmethoxycarbonyl amino acid pentafluorophenyl esters. The crude peptide, obtained after trifluoroacetic acid cleavage, was purified by gel filtration and by reverse-phase HPLC. This synthetic replicate, corresponding to human GIP1-31, retains the ability of naturally occurring GIP to stimulate insulin release.This publication has 2 references indexed in Scilit:
- The incretin concept todayDiabetologia, 1979
- A mild procedure for solid phase peptide synthesis: use of fluorenylmethoxycarbonylamino-acidsJournal of the Chemical Society, Chemical Communications, 1978