Redistribution of hydrogen ion and chloride, and water shift across the red cell membrane of human and ruminant blood with changes in oxygen saturation

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Abstract
On oxygenation of blood the distribution ratios of H+ and Cl− across red cell membrane are decreased and shift of water out of the red cell is produced. The magnitudes of these changes with 100% increase in oxygen saturation of blood were investigated for human and ruminant blood. A distribution ratio of H+, H plasma + /H+(DMO)cell=RH(DMO), was determined, in which H plasma + was measured by a pH electrode method and H+(DMO)cell, by the14C-DMO method. RH(DMO) was compared with the values of RCl and RaH obtained previously (Takano et al., 1976). RCl represented a distribution ratio of Cl− and RaH, a distribution ratio of H+ in which H plasma + and H cell + have been measured by a pH electrode method. Water shift was computed from a change in mean corpuscular hemoglobin concentration. On oxygenation RH(DMO), RCl and RaH were decreased in the magnitudes of 0.04–0.09 in human blood at plasma pH 7.40 and at 37° C. In ruminant blood the decreases in RH(DMO) and RCl were similar to those in human blood while that in RaH was not significant. On oxygenation the water shift produced was at a rate of 1.1–1.5% in human blood whilst in ruminant blood there was no significant shift. The results for water shift underlined the relevance of the results for RaH but not for RH(DMO) nor RCl in ruminant blood. The results for RaH and water shift suggest that the redistribution of H+, Cl− and water with oxygenation of blood is smaller in ruminant blood than in human blood.