Blood culture technique based on centrifugation: clinical evaluation

Publisher Website
Google Scholar
Abstract
Blood samples (1000) from suspected bacteremia patients were analyzed concurrently, where possible, by 3 methods: trypticase soy broth with sodium polyanethol sulfonate and a CO2 atmosphere, pour plates with brain heart infusion agar or Sabouraud dextrose agar, and centrifugation of the suspected organism in a hypertonic solution. There were 176 positive cultures. The centrifugation technique recovered 73% of the positive cultures. The broth and pour plate techniques recovered 38 and 49%, respectively. The centrifugation technique showed an increased isolation rate for Pseudomonas, fungi and gram-positive cocci. For each organism the time required for the detection of a positive culture was shortest for the centrifugation technique.