Identificationof Proteins Associated with Murine Gammaherpesvirus68Virions
Open Access
- 15 December 2003
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 77 (24) , 13425-13432
- https://doi.org/10.1128/jvi.77.24.13425-13432.2003
Abstract
Murine gammaherpesvirus 68 (MHV68 [also known asγ HV-68]) is distinguished by its ability to replicate to high titers in cultured cells, making it an excellent candidate for studying gammaherpesvirus virion composition. Extracellular MHV68 virions were isolated, and abundant virion-associated proteins were identified by mass spectrometry. Five nucleocapsid protein homologues, the tegument protein homologue encoded by open reading frame (ORF) 75c, and envelope glycoproteins B and H were detected. In addition, gene products from MHV68 ORF20, ORF24, ORF28, ORF45, ORF48, and ORF52 were identified in association with virions, suggesting that these gammaherpesvirus genes are involved in the early phase of infection or virion assembly and egress.Keywords
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