New Diagnostic Method for HPLC Colum

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Abstract
A new method for testing high-performance liquid chromatography columns is based on the experimentally found pressure dependence of preferential sorption of mixed eluent components on column filling materials. Sudden changes of pressure (e.g., due to column backflushing) produce changes in the composition of the liquid adsorbed on the sorbent surface, and consequently the composition of the effluent is altered. The “eigenzone” of the new composition leaving the column can easily be observed with a non-specific detector, such as a differential refractometer. From the shape of the eigenzone, the uniformity of the sorbent bed within the column can be judged, and the efficiency of the column-packing procedure can be determined. Column backflushing tests make heavy demands on the sorbent bed and improperly packed columns are destroyed. Well-packed columns, however, remain unaffected; thus, the column backflushing method can be used for testing column stability.

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