The Occurrence of a Strain of Azotobacter chroococcum Which Does Not Ferment Mannitol

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Abstract
A strain of Azotobacter chroococcum which could not use mannitol as a source of energy was isolated from various soils by substituting a carbohydrate such as dextrin, starch or sucrose for mannitol in Ashby''s agar. It has been designated as a mannitol-negative strain, since the only distinguishing characteristic seems to be its inability to utilize mannitol. In certain soil samples from N. Dakota, Utah and Colorado this mannitol-negative strain was the only Azotobacter present, whereas in other soil samples from these same states, its number varied from 0 to 70 per cent of the Azotobacter population. The mannitol-negative strain was also found in varying proportions in soils from Nebraska, Texas, Delaware, Hawaii, Chile and Greece. This strain was not found in soil from Washington, D. C, Jeanerette, Louisiana, Port Royal, Pennsylvania, and Turkey.

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