Carbohydrate Metabolism in Leukocytes VII. Metabolism of Glucose, Acetate, and Propionate by Human Plasma Cells

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Abstract
Plasma cells obtained from the peripheral blood of a patient with multiple myeloma was incubated in serum and Krebs-Ringer bicarbonate buffer with 14C-labeled glucose, acetate, and propionate. Glucose utilization by these cells amounted to 0.5 [mu] mole per hr per 108 cells and was mainly via the Embden-Meyerhof pathway and only 6 percent or less traversed the hexose monophosphate shunt. The presence of Krebs cycle activity was demonstrated by direct isolation of several labeled intermediates after incubation with either 14c-acetate or 14C-propionate. The distribution of 14C in lactate, succinate, fuma-rate, malate, aspartate, and glutamate indicate a complete Krebs cycle. Acetate was metabolized via the Krebs cycle to the extent of 0.15 [mu] moles per hr per 108 cells, and the rate of propionate utilization was 0.17[mu] moles per hr per 108 cells.