IGF-I and IGFBP-3 transport in the rat heart
- 1 January 2003
- journal article
- Published by American Physiological Society in American Journal of Physiology-Endocrinology and Metabolism
- Vol. 284 (1) , E237-E239
- https://doi.org/10.1152/ajpendo.00336.2002
Abstract
Specific binding of IGF-binding protein (IGFBP)-3 was shown to be present in the isolated, beating rat heart. The uptake of perfused 125I-labeled IGF-I in the beating heart was decreased to 9% by blocking IGF-I binding sites with the IGF-I analog Long R3 (LR3) IGF-I. When LR3 was perfused with complexes of125I-IGF-I · IGFBP-3, uptake of125I-IGF-I was decreased to 41%, which was significantly greater than LR3 and 125I-IGF-I (41 vs. 9%). These data suggest that both microvessel IGF-I and IGFBP-3 binding sites contribute to the transport of IGF-I in the perfused rat heart. This also suggests a novel and plausible mechanism whereby circulating IGFs reach sites of IGF bioactivity.Keywords
This publication has 12 references indexed in Scilit:
- Structure-function relationships of insulin-like growth factor binding protein 6 (IGFBP-6) and its chimeras.Growth Hormone & IGF Research, 2002
- Use of Mutagenesis to Probe IGF-Binding Protein Structure/Function RelationshipsEndocrine Reviews, 2001
- Use of Mutagenesis to Probe IGF-Binding Protein Structure/Function RelationshipsEndocrine Reviews, 2001
- Distribution of Chimeric IGF Binding Protein (IGFBP)-3 and IGFBP-4 in the Rat Heart: Importance of C-Terminal Basic RegionEndocrinology, 2001
- Distribution of Chimeric IGF Binding Protein (IGFBP)-3 and IGFBP-4 in the Rat Heart: Importance of C-Terminal Basic RegionEndocrinology, 2001
- Usefulness of Different Biochemical Markers of the Insulin-Like Growth Factor (IGF) Family in Diagnosing Growth Hormone Excess and Deficiency in AdultsJournal of Clinical Endocrinology & Metabolism, 2001
- The Combination of Insulin-Like Growth Factor I and Insulin-Like Growth Factor-Binding Protein-3 Reduces Insulin Requirements in Insulin-Dependent Type 1 Diabetes: Evidence for in VivoBiological ActivityJournal of Clinical Endocrinology & Metabolism, 2000
- INSULIN DIFFERENTIALLY ALTERS TRANSCAPILLARY MOVEMENT OF INTRAVASCULAR IGFBP-1, IGFBP-2 AND ENDOTHELIAL CELL IGF-BINDING PROTEINS IN THE RAT HEARTEndocrinology, 1990
- Vascular transport of insulin to rat cardiac muscle. Central role of the capillary endothelium.Journal of Clinical Investigation, 1988
- Receptor-Mediated Transport of Insulin Across Endothelial CellsScience, 1985