Temperature-sensitive replication of H5ts125 adenovirus DNA in vitro.
- 1 September 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (9) , 4291-4295
- https://doi.org/10.1073/pnas.75.9.4291
Abstract
A soluble adenovirus DNA replication system was prepared in extracts of [human cervical carcinoma] HeLa cells infected with the temperature-sensitive mutant H5ts125. These nuclear extracts synthesize full-sized viral DNA at 30.degree. C but are temperature sensitive in this function at 38.degree. C. A 72,000 dalton DNA-binding protein purified from cells infected with wild-type virus complements the temperature-sensitive defect and restores viral DNA replication to 74% of normal values.This publication has 32 references indexed in Scilit:
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