In Vivo Synthesis of Crown Gall-specific Agrobacterium tumefaciens-directed Derivatives of Basic Amino Acids

Publisher Website
Google Scholar
Abstract
Several kinds of primary sunflower (Helianthus annuus) crown gall tissues were established in tissue culture, then labeled in vivo with either [14C]arginine, [14C]histidine, [3H]lysine or [3H]ornithine. Crown gall tissues incited by A. tumefaciens strains that utilize octopine as a sole source of C or N for growth synthesized the 4 members of the N2-(1-carboxyethyl)-amino acid family: octopine, histopine, lysopine and octopinic acid. Those tissues incited by A. tumefaciens strains that utilize nopaline synthesized nopaline and 2 new compounds, a lysine and an ornithine derivative (ornaline). A normal tissue culture, a habituated tissue culture and a crown gall culture from a strain of the bacteria unable to utilize either octopine or nopaline did not synthesize any of the amino acid derivatives. No other crown gall-specific derivative of the 4 basic amino acids was detected.