A Method for the Detemination of Respiratory Quotient by Gas Chromatography

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Abstract
A gas chromatographic method for the determination of respiratory quotient was described. The system employed 2 columns in series, the first filled with Porapak Q separated CO2 from air, and the second packed with molecular sieve 5A separated O2 from N2. Each gas component was measured with a thermal conductivity detector; slight differences between the concentrations were detected and the reproducibility was good. Effects of the O2 concentration on the respiration of a wolf spider, Pardosa astrigera (L. Koch), were investigated. Reduction in the O2 concentration to 17-18% significantly depressed the respiration. The results suggest that the O2 concentration inside the respiratory chambers should not decrease below this critical value.

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