Simultaneous removal of sperm plasma membrane and acrosome before intracytoplasmic sperm injection improves oocyte activation/embryonic development
- 21 November 2006
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 103 (47) , 17661-17666
- https://doi.org/10.1073/pnas.0608183103
Abstract
Direct injection of a single spermatozoon into an oocyte (ICSI) can produce apparently normal offspring. Although the production of normal offspring by ICSI has been successful in mice and humans, it has been less successful in many other species. The reason for this is not clear, but could be, in part, due to inconsistent activation of oocytes because of delayed disintegration of sperm plasma membrane within oocytes and incorporation of the acrosome containing a spectrum of hydrolyzing enzymes. In the mouse, the removal of sperm plasma membrane and acrosome was not a prerequisite to produce offspring by ICSI, but it resulted in earlier onset of oocyte activation and better embryonic development. The best result was obtained when spermatozoa were demembranated individually immediately before ICSI by using lysolecithin, a hydrolysis product of membrane phospholipids.Keywords
This publication has 38 references indexed in Scilit:
- Thirty years of calcium signals at fertilizationSeminars in Cell & Developmental Biology, 2006
- Destabilization of the acrosome results in release of phospholipase A2 from human spermatozoa and subsequent formation of lysophospholipidsAndrologia, 2006
- Intracytoplasmic sperm injection in the bovine induces abnormal [Ca2+]i responses and oocyte activationReproduction, Fertility and Development, 2006
- Increased disruption of sperm plasma membrane at sperm immobilization promotes dissociation of perinuclear theca from sperm chromatin after intracytoplasmic sperm injection in pigsReproduction, 2005
- Ca2+ oscillation-inducing phospholipase C zeta expressed in mouse eggs is accumulated to the pronucleus during egg activationDevelopmental Biology, 2004
- The cytosolic sperm factor that triggers Ca2+ oscillations and egg activation in mammals is a novel phospholipase C: PLCζReproduction, 2004
- Patterns of Intracellular Calcium Oscillations in Horse Oocytes Fertilized by Intracytoplasmic Sperm Injection: Possible Explanations for the Low Success of This Assisted Reproduction Technique in the Horse1Biology of Reproduction, 2004
- Interactions of sperm perinuclear theca with the oocyte: Implications for oocyte activation, anti‐polyspermy defense, and assisted reproductionMicroscopy Research and Technique, 2003
- Production of motile acrosome-reacted mouse sperm with nanomolar concentration of calcium ionophore A23187Molecular Reproduction and Development, 1994
- Thermostability of Sperm Nuclei Assessed by Microinjection into Hamster Oocytes1Biology of Reproduction, 1991