A strategy for the identification of 2′-deoxynucleoside and 2′--deoxynucleotide adducts using electrospray tandem mass spectrometry

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Abstract
A methodology using electrospray tandem mass spectrometry (ES MS/MS) was developed to identify 2′-deoxynucleotide adducts of bisphenol A diglycidylether (BPADGE). The 2′-deoxynucleotide adducts were formed in vitro and were separated using reversed-phase high-performance liquid chromatography (RP-HPLC). The adducts were investigated by (–) electrospray liquid chromatography-mass spectrometry [(–) ES LC-MS]. (–) ES LC-MS/MS allowed the differentiation between 5′-phosphate- and base-alkylated adducts. More detailed structural information on the heterocyclic moiety was obtained by examining the (+) ES LC-MS/MS data and by comparison with the (+) ES LC-MS/MS spectra of the corresponding 2′-deoxynucleoside adducts.

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