Rapid, Simplified Continuous-Flow Colorimetry of Serum Triglycerides
Open Access
- 1 December 1973
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 19 (12) , 1391-1393
- https://doi.org/10.1093/clinchem/19.12.1391
Abstract
A new modified, semi-automated method for triglyceride assay is described. The sampling rate is rapid (60/h) with acceptable inter-sample wash. Color and triglyceride concentration are linearly related up to 500 mg/dl. The modifications were: heated reactions were eliminated, a short Kel-F coil was used for transesterification, arrangements were made for simultaneous debubbling and phase separating. Both comparison with another semi-automated procedure and recovery values indicate the acceptability of this proposed method for use in routine clinical laboratories.Keywords
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