HUMAN T-LYMPHOCYTE CELL LINE PRODUCING COLONY-STIMULATING ACTIVITY

Google Scholar
  • 1 January 1978
    • journal article
    • research article
    • Vol. 52  (5) , 1068-1072
Abstract
A permanent human T [thymus-derived] lymphocyte cell line that elaborates a potent colony-stimulating activity (CSA) was discussed. The line was established with spleen cells from a patient with a T lymphocyte variant of hairy-cell leukemia. These cells formed rosettes with sheep erythrocytes, showed a proliferative response to phytohemagglutinin [PHA] and were lysed by anti-thymocyte globulin. They did not synthesize immunoglobulin, nor did they contain Epstein-Barr virus. CSA was regularly detected in the supernatant medium after 3 days culture. With PHA there was augmented elaboration of CSA. Maximal activity was reached by 2 days and was 20% greater than that produced by a feeder layer of 1 .times. 106 peripheral blood leukocytes. One .mu.l of the supernatant material stimulated colony formation from the light-density nonadherent fraction of human bone marrow. There was maximal activity from 10-50 .mu.l/ml. Conditioned medium from these cells had little effect in stimulating CFU-C [granulocytic progenitor cells] from murine bone marrow. The availability of a human T lymphocyte line producing CSA will provide a source for large quantities of the lymphocyte-derived hormone and permit a definition of factors modulating the interaction of T lymphocytes with granulocyte and monocyte stem cells.