Sequence studies of nonradioactive Mycoplasma tRNAPhewith the aid of polynucleotide phosphorylase and polynucleotide kinase
- 1 January 1974
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 1 (12) , 1733-1738
- https://doi.org/10.1093/nar/1.12.1733
Abstract
The known methods of enzymatic phosphorylation with [(32)P]phosphate of the 3'- or 5'-hydroxyl group of an oligonucleotide have been applied to oligonucleotides derived from Mycoplasma tRNA(Phe). The fingerprints obtained by both methods are very similar to each other and to that of uniformly labelled tRNA. The sequence of some oligonucleotides was determined by partial digestion of the 3'-phosphorylated fragment with spleen phosphodiesterase and of the corresponding 5'-phosphorylated fragment with venom phosphodiesterase.Keywords
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