Brief Communication : Several Changes Associated With the Acquisition of a Single Chromosome in Rat Glial Tumor Cells 2

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Abstract
Two subcultures of a cell line, derived from a rat glial tumor, diverged in their properties when grown in medium containing different concentrations of fetal calf serum. Changes were noted in their morphology, response to treatment with dibutyryl cyclic adenosine monophosphate, and plating efficiency in soft agar. One subculture had a less “transformed” phenotype than the other. This culture also underwent a change in karyotype: It no longer had the diploid number of 42 chromosomes but a modal number of 43 chromosomes. Both subcultures were tumorigenic and gave histologically identical tumors with a similar latent period. A tumor derived from the less transformed culture had a modal chromosome number of 42. Thus possibly transformed properties in both cultures were expressed only by cells with a 42-chromosome karyotype. Reversal of the initial change in serum concentration had no further effect, the subcultures remaining true to their parental type.

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