A Modification of the Alcian Blue Method for Staining Hyaluronic Acid Substances in Tissue Sections

Publisher Website
Google Scholar
Abstract
Hyaluronic acid films and paraffin sections of open wounds were stained with a 1% solution of Alcian blue 8GS at pH ranges between 4.0 and 2.5. Tissues were treated with Streptomyces hyaluronidase prior to staining. Untreated sections showed that material stained with Alcian blue 8GS at pH 4.0 was of increased intensity when compared to sections stained at pH 2.5. Hyaluronic acid films stained faintly at pH 2.5 and when treated with enzyme. Early wound tissue considered to be a rich source of hyaluronic acid stained intensely with Alcian blue 8GS at pH 4.0 and with decreased intensity at pH 2.5 and when digested with Streptomyces hyaluronidase. The use of Alcian blue 8GS at pH 4.0 is recommended for the study of connective tissues rich in hyaluronic acid material.

This publication has 0 references indexed in Scilit: