COMPLEMENT-FIXING + NEUTRALIZING ANTIBODY RESPONSE TO BOVINE VIRAL DIARRHEA + HOG CHOLERA ANTIGENS

  • 1 January 1964
    • journal article
    • research article
    • Vol. 28  (1) , 19-+
Abstract
In calves inoculated with bovine viral diarrhea (BVD) viruses and soluble antigen, the complement-fixing (CF) antibodies appeared before serum-neutralizing (SN) antibodies and remained at high levels throughout the test period. A rapid rise in SN antibodies occurred after challenge with homologous virus with no apparent effect on CF antibody levels. The CF antibody responses in calves infected with cytopathogenic NADL-MD and non-cytopathogenic CG-1220 viruses were similar whereas SN antibody responses indicated strain specificity by reciprocal cross-neutralization tests. The CF antibody levels in 5 hog cholera (HC) antisera were assayed using the soluble antigen of NADL-MD BVD virus. No demonstrable SN antibodies were present in four HC antisera tested against NADL-MD virus, but a significant titer was present in the commercially prepared antiserum. Virus was reisolated from animals infected with BVD viruses by buffy coat culture technique during 3 weeks postinoculation, even when significant levels of CF and SN antibodies were present.