The interaction of normal lymphocytes and cells from lymphoid cell lines. I. The nature of the activation process.

  • 1 August 1970
    • journal article
    • Vol. 19  (2) , 329-42
Abstract
Human peripheral lymphocytes from all donors tested were mitotically activated when cultured in the presence of X-irradiated cells from continuously cultivated lymphoid cell lines (LCL). X-irradiated cells from LCL originating from normal individuals were effective as well as those of Burkitt's tumour origin and cells from LCL reported to be free of herpes-like virus were as effective as those reported to contain virus. The activation was of much greater intensity than the normal mixed lymphocyte reaction but occurred over a similar culture period. The reaction occurred only under conditions permitting direct cell contact between the two populations of cells. Most stimulation was obtained with viable X-irradiated LCL cells. Disrupted LCL cells were ineffective.