Enrichment and screening of heat-sensitive mutants ofPhysarum polycephalum
- 1 February 1978
- journal article
- research article
- Published by Hindawi Limited in Genetics Research
- Vol. 31 (1) , 1-12
- https://doi.org/10.1017/s0016672300017766
Abstract
SUMMARY: A new method for the isolation of temperature-sensitive mutants ofPhysarum polycephalumis described. It involves enrichment and prescreening of mutagenized amoebae followed by screening at both the plasmodial and amoebal stage. A total of 74 temperature-sensitive strains were recovered of which 26 were temperature-sensitive only as plasmodia, 35 only as amoebae and 13 in both stages. After a shift to the nonpermissive temperature, DNA and protein synthesis were followed in temperature-sensitive plasmodia to discover if the lesion affected functions of the nuclear cycle.This publication has 13 references indexed in Scilit:
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