REJECTION AND ENHANCEMENT IN AN IN VITRO MODEL OF ALLOIMMUNITY

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Abstract
SUMMARY A 4-hr quantitative assay for alloimmune lymphocyte-mediated cytotoxicity (LMC) using 51Cr-labeled thymocyte target cells has been developed and utilized to study the mechanisms allowing successful hybrid, but not parental, immunologically enhanced renal allografts in the rat. The hybrid cell was found to be more resistant to LMC and the cytolytic action of cytotoxic antibody than the parental cell. Furthermore, it was found that antitarget cell enhancing antibody was more effective in abrogating LMC mounted against the hybrid than the parental cell. Although the histocompatibility site densities of hybrid and parental cells are unknown, these data suggest that the parental cells have higher densities of histoincompatible sites, that F1 cells are heterogeneous with respect to expression of parental antigens, and that such alloantigen site densities underlie the differences in parental to parental and F1 hybrid to parental graft rejection.

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