Control of Ovulation Rate and Fertilization in Prepuberal Gilts

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Abstract
Prepuberal gilts from 154 to 180 days of age were injected with 250, 500, 1,000 or 2,000 I.U. of pregnant mare serum (PMS).The gilts were injected with human chorionic gonadotrophin (HCG) and artificially inseminated 48 and 72 hr., respectively, after the PMS injection. All 40 gonadotrophin treated gilts ovulated. The number of ovulations increased linearly (P<.05) with increased doses of PMS. The level of PMS had no effect on the percent of eggs recovered or the percent of fertilized eggs. However, the number of gilts containing fertilized eggs, the number of immature eggs and the number of polyspermic eggs increased significantly (P<.05) with increased doses of PMS. Two or more eggs were recovered from each gilt for a total of 660 eggs and a recovery rate of 81%. Fertilized eggs were recovered from all 22 gilts which showed spermatozoan transport. Within these females, 301 eggs (76%)were undergoing normal development, whereas 43 (6%) were polyspermic and 16 (4%) showed some degree of fragmentation. A total of 48 (7%) of the eggs contained vesicular nuclei and were therefore classified as immature. Sixty-three percent of the immature eggs were from gilts showing good sperm transport and were polyspermic. The number of sperm in the ooplasm of the immature eggs was significantly (P<.01) correlated with the number of sperm in the perivitelline space and on the zona pellucida. Immature eggs recovered from gilts showing sperm transport contained significantly(P<.05) more sperm than the normal fertilized eggs. Copyright © 1968. American Society of Animal Science. Copyright 1968 by American Society of Animal Science

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