Novel insertion sequence-like elements in phytoplasma strains of the aster yellows group are putative new members of the IS3 family
Open Access
- 1 January 2005
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 242 (2) , 353-360
- https://doi.org/10.1016/j.femsle.2004.11.036
Abstract
Novel insertion sequence (IS)-like elements were isolated and characterized from phytoplasma strains in the aster yellows (AY) group (16SrI). The IS-like elements were cloned from phytoplasma strains AY1 and NJAY or PCR-amplified from 15 additional strains representing nine subgroups in the AY group using primers based on sequences of the putative transposases (Tpases). All IS-like elements contained sequences encoding similar Tpases of 321 amino acids (320 for strain CPh). Substantial amino acid sequence variability suggested multiple species of Tpases or IS-like elements exist in the AY phytoplasma group. These Tpases have an identical DDE motif that is most similar to the DDE consensus of Tpases in the IS3 family.Keywords
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