Manometric determination of l-aspartic acid in l-asparagine

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Abstract
Manometric methods for the detn. of L-aspartic acid, L-asparagine and alpha-ketoglutaric acid, based on the use of aspartic-glutamic transaminase, oxaloacetic decarboxylase and glutamic decarboxylase, are described. The methods were applied to the analysis of casein, animal tissues, homogenates from animal tissues and plant material. A rapid conversion of glutamic acid into aspartic acid occurred in respiring liver homogenates. Data on the changes in the content of aspartic acid and glutamic acid and their amides in growing etiolated pea seedlings are given. Data are also given on the concns. of aspartic acid, asparagine and alpha-ketoglutaric acid in animal tissues and on the changes in these concns. which occur soon after death as a result of autolysis.