Unique organization of the human BCR gene promoter
- 1 January 1990
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 18 (23) , 7119-7125
- https://doi.org/10.1093/nar/18.23.7119
Abstract
The promoter of the human BCR gene, regulating the transcription of the chimeric BCR/ABL mRNA in leukemia, has been isolated and characterized. A region of 1.1 kb immediately 5' to the transcription start site was analyzed in detail by sequencing, DNase 1 footprinting, gel retardation and functional studies. These experiments localized a minimal promoter to a 650 bp sequence, composed of 270 bp of 5' flanking sequences and 380 bp of exon 1 transcribed sequences. The promoter region includes a TTTAA box, one Sp1 site and a novel protein-binding sequence absolutely necessary for efficient transcription in vivo. Six additional protein-binding regions were identified more to the 5'. Of these, one is found in an inverted repeat in the 3' coding and splice donor region of BCR exon 1.Keywords
This publication has 21 references indexed in Scilit:
- IDENTIFICATION OF 2 NORMAL BCR GENE-PRODUCTS IN THE CYTOPLASM1988
- Unique Forms of the abl Tyrosine Kinase Distinguish Ph 1 -Positive CML from Ph 1 -Positive ALLScience, 1987
- Overlapping cDNA clones define the complete coding region for the P210c-abl gene product associated with chronic myelogenous leukemia cells containing the Philadelphia chromosome.Proceedings of the National Academy of Sciences, 1986
- The chronic myelocytic cell line K562 contains a breakpoint in bcr and produces a chimeric bcr/c-abl transcript.Molecular and Cellular Biology, 1986
- Soviet alcoholism: Drunk driver stripped of degreeNature, 1985
- Fused transcript of abl and bcr genes in chronic myelogenous leukaemiaNature, 1985
- CHRONIC MYELOGENOUS LEUKEMIA - RECENT ADVANCES1985
- The human cellular abl gene product in the chronic myelogenous leukemia cell line K562 has an associated tyrosine protein kinase activityVirology, 1985
- An alteration of the human c-abl protein in K562 leukemia cells unmasks associated tyrosine kinase activityCell, 1984
- The Ph1Chromosome in Childhood LeukaemiaBritish Journal of Haematology, 1979